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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®594-conjugated BCL2 Recombinant antibody (CL594-80313)

    IF Staining of human appendicitis using CL594-80313

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®594 BCL2 antibody (CL594-80313, Clone: 3O5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated BCL2 Recombinant antibody (CL594-80313)

    FC experiment of Jurkat using CL594-80313

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BCL2 (CL594-80313, Clone:3O5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated BCL2 Recombinant antibody

BCL2 Recombinant Antibody for IF-P, FC (Intra)
Cat No. CL594-80313
Clone No.3O5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P, FC (Intra)

Synonyms

BCL-2, 3O5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80313 targets BCL2 in IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL2 fusion protein Ag3508 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2934803
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).

Protocols

Product Specific Protocols
IF protocol for CL594 BCL2 antibody CL594-80313Download protocol
Standard Protocols
Click here to view our Standard Protocols