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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL6 Recombinant antibody, PBS Only

BCL6 Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K6

Cat no : 81754-1-PBS

Synonyms

BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL6 Recombinant antibody (81754-1-RR)

    WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Ramos cells using BCL6 Recombinant antibody (81754-1-RR)

    IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    IP result of anti-BCL6 (IP:81754-1-RR, 4ug; Detection:81754-1-RR 1:8000) with Ramos cells lysate 1680 ug. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL6 Recombinant antibody (81754-1-RR)

    IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL6 Recombinant antibody (81754-1-RR)

    IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Ramos cells using BCL6 Recombinant antibody (81754-1-RR)

    FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BCL6 (81754-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81754-1-RR

    Affinity and Kinetic Characterization of 81754-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81754-1-RR against Human BCL6 were performed. The affinity constant is 0.157 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81754-1-PBS targets BCL6 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL6 fusion protein Ag15519 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 6
Calculated molecular weight 706 aa, 79 kDa
Observed molecular weight 79-95 kDa
GenBank accession numberBC150184
Gene symbol BCL6
Gene ID (NCBI) 604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.