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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL6 Recombinant antibody

BCL6 Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

3K6

Cat no : 81754-1-RR

Synonyms

BCL-6, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, B cell lymphoma 6 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL6 Recombinant antibody (81754-1-RR)

    WB analysis using 81754-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Ramos cells using BCL6 Recombinant antibody (81754-1-RR)

    IP experiment of Ramos using 81754-1-RR

    IP result of anti-BCL6 (IP:81754-1-RR, 4ug; Detection:81754-1-RR 1:8000) with Ramos cells lysate 1680 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL6 Recombinant antibody (81754-1-RR)

    IHC staining of human tonsillitis using 81754-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL6 Recombinant antibody (81754-1-RR)

    IHC staining of human tonsillitis using 81754-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Ramos cells using BCL6 Recombinant antibody (81754-1-RR)

    FC experiment of Ramos using 81754-1-RR

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BCL6 (81754-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81754-1-RR

    Affinity and Kinetic Characterization of 81754-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81754-1-RR against Human BCL6 were performed. The affinity constant is 0.157 nM.

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Tested Applications

Positive WB detected inRamos cells, Daudi cells
Positive IP detected inRamos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81754-1-RR targets BCL6 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL6 fusion protein Ag15519 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 6
Calculated molecular weight 706 aa, 79 kDa
Observed molecular weight 79-95 kDa
GenBank accession numberBC150184
Gene symbol BCL6
Gene ID (NCBI) 604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Protocols

Product Specific Protocols
WB protocol for BCL6 antibody 81754-1-RRDownload protocol
IHC protocol for BCL6 antibody 81754-1-RRDownload protocol
IP protocol for BCL6 antibody 81754-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols