CoraLite® Plus 488-conjugated BCL6 Recombinant antibody
BCL6 Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3K6
Cat no : CL488-81754
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Ramos cells |
| Positive FC detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-81754 targets BCL6 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | BCL6 fusion protein Ag15519 相同性解析による交差性が予測される生物種 |
| Full Name | B-cell CLL/lymphoma 6 |
| Calculated molecular weight | 706 aa, 79 kDa |
| Observed molecular weight | 79-95 kDa |
| GenBank accession number | BC150184 |
| Gene symbol | BCL6 |
| Gene ID (NCBI) | 604 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 BCL6 antibody CL488-81754 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
