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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCL9 Recombinant antibody (83760-3-RR)

    IF Staining of HeLa using 83760-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (83760-3-RR, Clone: 240682A9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using BCL9 Recombinant antibody (83760-3-RR)

    FC experiment of SH-SY5Y using 83760-3-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Bcl9 Recombinant Antibody (83760-3-RR, Clone:240682A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83760-3-RR

    Affinity and Kinetic Characterization of 83760-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83760-3-RR against Human BCL9 were performed. The affinity constant is 5.39 nM.

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BCL9 Recombinant antibody

BCL9 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83760-3-RR
Clone No.240682A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83760-3-RR targets BCL9 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL9 fusion protein Ag19158 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 9
Calculated molecular weight 1426 aa, 149 kDa
GenBank accession numberBC116451
Gene Symbol BCL9
Gene ID (NCBI) 607
RRIDAB_3671356
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Protocols

Product Specific Protocols
IF protocol for BCL9 antibody 83760-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols