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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83760-3 targets BCL9 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | BCL9 fusion protein Ag19158 相同性解析による交差性が予測される生物種 |
| Full Name | B-cell CLL/lymphoma 9 |
| Calculated molecular weight | 1426 aa, 149 kDa |
| GenBank accession number | BC116451 |
| Gene Symbol | BCL9 |
| Gene ID (NCBI) | 607 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00512 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BCL9 antibody CL488-83760-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |