Validation Data Gallery
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Tested Applications
| Positive WB detected in | Colo320 cells, COLO 320 cells, HEK-293 cells |
| Positive IHC detected in | human gliomas tissue, human brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60212-1-Ig targets BCS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BCS1L fusion protein Ag18280 相同性解析による交差性が予測される生物種 |
| Full Name | BCS1-like (yeast) |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC007500 |
| Gene Symbol | BCS1L |
| Gene ID (NCBI) | 617 |
| RRID | AB_11042318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y276 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BCS1L antibody 60212-1-Ig | Download protocol |
| IHC protocol for BCS1L antibody 60212-1-Ig | Download protocol |
| IF protocol for BCS1L antibody 60212-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |