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  • Featured Product
  • KD/KO Validated

BCS1L Monoclonal antibody, PBS Only (Capture)

BCS1L Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G6H1

Cat no : 60212-1-PBS

Synonyms

h-BCS1, hBCS1, BCS1-like protein, BCS1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using BCS1L Monoclonal antibody (60212-1-Ig)

    WB analysis of HEK-293 using 60212-1-Ig (same clone as 60212-1-PBS)

    WB result of BCS1L antibody (60212-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using BCS1L Monoclonal antibody (60212-1-Ig)

    WB analysis of COLO 320 using 60212-1-Ig (same clone as 60212-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60212-1-Ig (BCS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BCS1L Monoclonal antibody (60212-1-Ig)

    IHC staining of human gliomas using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BCS1L Monoclonal antibody (60212-1-Ig)

    IHC staining of human gliomas using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BCS1L Monoclonal antibody (60212-1-Ig)

    IHC staining of human kidney using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BCS1L Monoclonal antibody (60212-1-Ig)

    IHC staining of human kidney using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using BCS1L Monoclonal antibody (60212-1-Ig)

    IHC staining of human brain using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using BCS1L Monoclonal antibody (60212-1-Ig)

    IHC staining of human brain using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BCS1L Monoclonal antibody (60212-1-Ig)

    IF Staining of MCF-7 using 60212-1-Ig (same clone as 60212-1-PBS)

    Immunofluorescent analysis of () fixed MCF-7 cells using 60212-1-Ig (BCS1L antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 60212-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50678-1

    Cytometric bead array standard curve of MP50678-1

    Cytometric bead array standard curve of MP50678-1, BCS1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60212-1-PBS. Detection antibody: 60212-2-PBS. Standard:Ag18280. Range: 1.563-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60212-1-PBS targets BCS1L as part of a matched antibody pair:

MP50678-1: 60212-1-PBS capture and 60212-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCS1L fusion protein Ag18280 相同性解析による交差性が予測される生物種
Full Name BCS1-like (yeast)
Calculated molecular weight 48 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007500
Gene symbol BCS1L
Gene ID (NCBI) 617
RRIDAB_11042318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.