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BDH1 Polyclonal antibody
BDH1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 15417-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, mouse liver tissue, rat liver tissue, HT-29 cells, human liver tissue, rat brain tissue, COLO 320 cells, mouse brain tissue, mouse heart tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | rat brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 23 publications below |
IHC | See 4 publications below |
IF | See 1 publications below |
Product Information
15417-1-AP targets BDH1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BDH1 fusion protein Ag7705 相同性解析による交差性が予測される生物種 |
Full Name | 3-hydroxybutyrate dehydrogenase, type 1 |
Calculated molecular weight | 38 kDa |
Observed molecular weight | 31 kDa |
GenBank accession number | BC005844 |
Gene symbol | BDH1 |
Gene ID (NCBI) | 622 |
RRID | AB_2274683 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
Protocols
Product Specific Protocols | |
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WB protocol for BDH1 antibody 15417-1-AP | Download protocol |
IHC protocol for BDH1 antibody 15417-1-AP | Download protocol |
IF protocol for BDH1 antibody 15417-1-AP | Download protocol |
IP protocol for BDH1 antibody 15417-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
J Cell Biol CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. |