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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BDH1 Polyclonal antibody

BDH1 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15417

Synonyms

Short chain dehydrogenase/reductase family 9C member 1, SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 488-conjugated BDH1 Polyclonal anti (CL488-15417)

    IF Staining of mouse liver using CL488-15417

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 BDH1 antibody (CL488-15417) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated BDH1 Polyclonal anti (CL488-15417)

    FC experiment of HepG2 using CL488-15417

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human BDH1 (CL488-15417) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15417 targets BDH1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BDH1 fusion protein Ag7705 相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 1
Calculated molecular weight 38 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC005844
Gene symbol BDH1
Gene ID (NCBI) 622
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BDH1 antibody CL488-15417Download protocol
FC protocol for CL Plus 488 BDH1 antibody CL488-15417Download protocol
Standard Protocols
Click here to view our Standard Protocols