Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig liver tissue, HSC-T6 cells, HepG2 cells, HT-29 cells, COLO 320 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
67448-1-Ig targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BDH1 fusion protein Ag7864 相同性解析による交差性が予測される生物種 |
| Full Name | 3-hydroxybutyrate dehydrogenase, type 1 |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC005844 |
| Gene Symbol | BDH1 |
| Gene ID (NCBI) | 622 |
| RRID | AB_2882682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02338 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| IHC protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| IF protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
MedComm (2020) BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression
| ||
bioRxiv An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells
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