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  • KD/KO Validated

BDH1 Monoclonal antibody

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67448-1-Ig
Clone No.2C3D4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (2)

Applications

WB, IHC, IF-P, ELISA

BDH, BDH1, SDR9C1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inpig liver tissue, HSC-T6 cells, HepG2 cells, HT-29 cells, COLO 320 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67448-1-Ig targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BDH1 fusion protein Ag7864 相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 1
Calculated molecular weight 38 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC005844
Gene Symbol BDH1
Gene ID (NCBI) 622
RRIDAB_2882682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02338
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Protocols

Product Specific Protocols
WB protocol for BDH1 antibody 67448-1-IgDownload protocol
IHC protocol for BDH1 antibody 67448-1-IgDownload protocol
IF protocol for BDH1 antibody 67448-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

MedComm (2020)

BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression

Authors - Jingjing Huang
  • KD Validated
human,mouseWB

bioRxiv

An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells

Authors - Faith C Kaluba
  • KD Validated
mouseWB

PNAS Nexus

Frataxin controls ketone body metabolism through regulation of OXCT1

Authors - Yi NA Dong
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