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  • Featured Product
  • KD/KO Validated

BDH1 Monoclonal antibody, PBS Only

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2C3D4

Cat no : 67448-1-PBS

Synonyms

SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using BDH1 Monoclonal antibody (67448-1-Ig)

    WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using BDH1 Monoclonal antibody (67448-1-Ig)

    WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using BDH1 Monoclonal antibody (67448-1-Ig)

    WB analysis of HepG2 using 67448-1-Ig (same clone as 67448-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using BDH1 Monoclonal antibody (67448-1-Ig)

    WB analysis of HT-29 using 67448-1-Ig (same clone as 67448-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using BDH1 Monoclonal antibody (67448-1-Ig)

    WB analysis of COLO 320 using 67448-1-Ig (same clone as 67448-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BDH1 Monoclonal antibody (67448-1-Ig)

    IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BDH1 Monoclonal antibody (67448-1-Ig)

    IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using BDH1 Monoclonal antibody (67448-1-Ig)

    IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using BDH1 Monoclonal antibody (67448-1-Ig)

    IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67448-1-PBS targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BDH1 fusion protein Ag7864 相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 1
Calculated molecular weight 38 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC005844
Gene symbol BDH1
Gene ID (NCBI) 622
RRIDAB_2882682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).