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BDH2 Monoclonal antibody

BDH2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6H5

Cat no : 68279-1-Ig

Synonyms

BDH2, DHRS6, EFA6R, FLJ13261, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BDH2 Monoclonal antibody (68279-1-Ig)

    WB analysis of HeLa using 68279-1-Ig

    WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using BDH2 Monoclonal antibody (68279-1-Ig)

    WB analysis using 68279-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BDH2 Monoclonal antibody (68279-1-Ig)

    IF Staining of HepG2 using 68279-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68279-1-Ig targets BDH2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BDH2 fusion protein Ag26108 相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Observed molecular weight 27 kDa
GenBank accession numberBC095414
Gene symbol BDH2
Gene ID (NCBI) 56898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.

Protocols

Product Specific Protocols
WB protocol for BDH2 antibody 68279-1-IgDownload protocol
IF protocol for BDH2 antibody 68279-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols