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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BDH2 Monoclonal antibody

BDH2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6H5

Cat no : CL488-68279

Synonyms

EC:1.1.1.-, DHRS6, 4-oxo-L-proline reductase, 3-hydroxybutyrate dehydrogenase type 2, 1B6H5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated BDH2 Monoclonal anti (CL488-68279)

    IF Staining of HepG2 using CL488-68279

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 BDH2 antibody (CL488-68279, Clone: 1B6H5 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68279 targets BDH2 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BDH2 fusion protein Ag26108 相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Observed molecular weight 27 kDa
GenBank accession numberBC095414
Gene symbol BDH2
Gene ID (NCBI) 56898
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BDH2 antibody CL488-68279Download protocol
Standard Protocols
Click here to view our Standard Protocols