- Featured Product
- KD/KO Validated
HRP-conjugated BID Monoclonal antibody
BID Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB
Conjugate
HRP
CloneNo.
2A2E5
Cat no : HRP-60301
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
HRP-60301 targets BID in WB applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | BID fusion protein Ag21005 相同性解析による交差性が予測される生物種 |
Full Name | BH3 interacting domain death agonist |
Calculated molecular weight | 22 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | BC009197 |
Gene symbol | BID |
Gene ID (NCBI) | 637 |
RRID | AB_2883832 |
Conjugate | HRP |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HRP BID antibody HRP-60301 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |