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BIN1 Polyclonal antibody
BIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 14647-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse skeletal muscle tissue, mouse brain tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human osteosarcoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
14647-1-AP targets BIN1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BIN1 fusion protein Ag6240 相同性解析による交差性が予測される生物種 |
| Full Name | bridging integrator 1 |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 50-65 kDa |
| GenBank accession number | BC004101 |
| Gene symbol | BIN1 |
| Gene ID (NCBI) | 274 |
| RRID | AB_2243396 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BIN1 antibody 14647-1-AP | Download protocol |
| IHC protocol for BIN1 antibody 14647-1-AP | Download protocol |
| IF protocol for BIN1 antibody 14647-1-AP | Download protocol |
| IP protocol for BIN1 antibody 14647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Neurodegener BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia. | ||
Int J Cancer Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients. | ||
Mol Biol Cell Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.
| ||
Toxicol Appl Pharmacol Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells. | ||
J Neuropathol Exp Neurol Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy. | ||
J Comp Neurol Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH CaX (Verified Customer) (10-24-2023) | An excellent Ab to probe Bin1 at the right MW.
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