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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BIN1 Polyclonal antibody

BIN1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14647

Synonyms

AMPH2, Amphiphysin II, Amphiphysin like protein, AMPHL, BIN1, bridging integrator 1, DKFZp547F068, SH3P9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated BIN1 Polyclonal anti (CL488-14647)

    IF Staining of mouse brain using CL488-14647

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 BIN1 antibody (CL488-14647) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14647 targets BIN1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN1 fusion protein Ag6240 相同性解析による交差性が予測される生物種
Full Name bridging integrator 1
Calculated molecular weight 65 kDa
Observed molecular weight 50-65 kDa
GenBank accession numberBC004101
Gene symbol BIN1
Gene ID (NCBI) 274
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BIN1 antibody CL488-14647Download protocol
Standard Protocols
Click here to view our Standard Protocols