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  • Featured Product
  • KD/KO Validated

BIN1 Polyclonal antibody, PBS Only

BIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14647-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BIN1 Polyclonal antibody (14647-1-AP)

    WB analysis using 14647-1-AP (same clone as 14647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BIN1 Polyclonal antibody (14647-1-AP)

    WB analysis of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using BIN1 Polyclonal antibody (14647-1-AP)

    WB analysis of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using BIN1 Polyclonal antibody (14647-1-AP)

    WB analysis of Jurkat using 14647-1-AP (same clone as 14647-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IP experiment of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    IP result of anti-BIN1 (IP:14647-1-AP, 4ug; Detection:14647-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human osteosarcoma tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human osteosarcoma tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using BIN1 Polyclonal antibody (14647-1-AP)

    IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14647-1-PBS targets BIN1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN1 fusion protein Ag6240 相同性解析による交差性が予測される生物種
Full Name bridging integrator 1
Calculated molecular weight 65 kDa
Observed molecular weight 50-65 kDa
GenBank accession numberBC004101
Gene symbol BIN1
Gene ID (NCBI) 274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).