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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of human spleen using 66953-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of Ramos using 66953-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of pig spleen using 66953-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of Raji using 66953-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of Daudi using 66953-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of human appendicitis using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of human appendicitis using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of mouse thymus using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of mouse thymus using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of rat spleen using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of rat spleen using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using BLNK Monoclonal antibody (66953-1-Ig)

    IF Staining of Raji using 66953-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using BLNK Monoclonal antibody (66953-1-Ig)

    IF Staining of NIH/3T3 using 66953-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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BLNK Monoclonal antibody

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66953-1-Ig
Clone No.1A1A1

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue, Raji cells, Daudi cells, Ramos cells, pig spleen tissue
Positive IHC detected inhuman appendicitis tissue, mouse thymus tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66953-1-Ig targets BLNK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BLNK fusion protein Ag28673 相同性解析による交差性が予測される生物種
Full Name B-cell linker
Calculated molecular weight 50 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC018906
Gene Symbol BLNK
Gene ID (NCBI) 29760
RRIDAB_2882276
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WV28
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

Protocols

Product Specific Protocols
WB protocol for BLNK antibody 66953-1-IgDownload protocol
IHC protocol for BLNK antibody 66953-1-IgDownload protocol
IF protocol for BLNK antibody 66953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols