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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of human spleen using 66953-1-Ig (same clone as 66953-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of Raji using 66953-1-Ig (same clone as 66953-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of Daudi using 66953-1-Ig (same clone as 66953-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of Ramos using 66953-1-Ig (same clone as 66953-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    WB analysis of pig spleen using 66953-1-Ig (same clone as 66953-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of mouse thymus using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of mouse thymus using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of rat spleen using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using BLNK Monoclonal antibody (66953-1-Ig)

    IHC staining of rat spleen using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using BLNK Monoclonal antibody (66953-1-Ig)

    IF Staining of Raji using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using BLNK Monoclonal antibody (66953-1-Ig)

    IF Staining of NIH/3T3 using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

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BLNK Monoclonal antibody, PBS Only

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66953-1-PBS
Clone No.1A1A1

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66953-1-PBS targets BLNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Reactivity Human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BLNK fusion protein Ag28673 相同性解析による交差性が予測される生物種
Full Name B-cell linker
Calculated molecular weight 50 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC018906
Gene Symbol BLNK
Gene ID (NCBI) 29760
RRIDAB_2882276
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WV28
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.