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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using BLZF1 Polyclonal antibody (16354-1-AP)

    WB analysis of K-562 using 16354-1-AP (same clone as 16354-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using BLZF1 Polyclonal antibody (16354-1-AP)

    WB analysis of Jurkat using 16354-1-AP (same clone as 16354-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using BLZF1 Polyclonal antibody (16354-1-AP)

    WB analysis of Jurkat using 16354-1-AP (same clone as 16354-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of mouse liver using 16354-1-AP (same clone as 16354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of mouse liver using 16354-1-AP (same clone as 16354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

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BLZF1 Polyclonal antibody, PBS Only

BLZF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16354-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16354-1-PBS targets BLZF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLZF1 fusion protein Ag9564 相同性解析による交差性が予測される生物種
Full Name basic leucine zipper nuclear factor 1
Calculated molecular weight 400 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC020716
Gene Symbol BLZF1
Gene ID (NCBI) 8548
RRIDAB_2918045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.