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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BMPR1A Recombinant antibody, PBS Only

BMPR1A Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230137B7

Cat no : 82928-1-PBS

Synonyms

ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMPR1A Recombinant antibody (82928-1-RR)

    WB analysis using 82928-1-RR (same clone as 82928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82928-1-RR (BMPR1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82928-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BMPR1A Recombinant antibody (82928-1-RR)

    IP experiment of HEK-293 using 82928-1-RR (same clone as 82928-1-PBS)

    IP result of anti-BMPR1A (IP:82928-1-RR, 4ug; Detection:82928-1-RR 1:2000) with HEK-293 cells lysate 1920 ug. This data was developed using the same antibody clone with 82928-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using BMPR1A Recombinant antibody (82928-1-RR)

    FC experiment of MCF-7 using 82928-1-RR (same clone as 82928-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BMPR1A Recombinant antibody (82928-1-RR, Clone:230137B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82928-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82928-1-RR

    Affinity and Kinetic Characterization of 82928-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82928-1-RR against Human BMPR1A were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82928-1-PBS targets BMPR1A in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BMPR1A fusion protein Ag3410 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type IA
Calculated molecular weight 532 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC028383
Gene symbol BMPR1A
Gene ID (NCBI) 657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).