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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BMPR1A Recombinant antibody

BMPR1A Recombinant Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230137B7

Cat no : 82928-1-RR

Synonyms

ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3, Activin receptor like kinase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMPR1A Recombinant antibody (82928-1-RR)

    WB analysis using 82928-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82928-1-RR (BMPR1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BMPR1A Recombinant antibody (82928-1-RR)

    IP experiment of HEK-293 using 82928-1-RR

    IP result of anti-BMPR1A (IP:82928-1-RR, 4ug; Detection:82928-1-RR 1:2000) with HEK-293 cells lysate 1920 ug.

  • Flow cytometry (FC) experiment of MCF-7 cells using BMPR1A Recombinant antibody (82928-1-RR)

    FC experiment of MCF-7 using 82928-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BMPR1A Recombinant antibody (82928-1-RR, Clone:230137B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82928-1-RR

    Affinity and Kinetic Characterization of 82928-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82928-1-RR against Human BMPR1A were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inJurkat cells, U2OS cells, HEK-293 cells, mouse heart tissue
Positive IP detected inHEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82928-1-RR targets BMPR1A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BMPR1A fusion protein Ag3410 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type IA
Calculated molecular weight 532 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC028383
Gene symbol BMPR1A
Gene ID (NCBI) 657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

Protocols

Product Specific Protocols
WB protocol for BMPR1A antibody 82928-1-RRDownload protocol
IP protocol for BMPR1A antibody 82928-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols