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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMPR2 Monoclonal antibody (68497-1-Ig)

    WB analysis using 68497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68497-1-Ig (BMPR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BMPR2 Monoclonal antibody (68497-1-Ig)

    WB analysis of NIH/3T3 using 68497-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68497-1-Ig (BMPR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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BMPR2 Monoclonal antibody

BMPR2 Monoclonal Antibody for WB, ELISA
Cat No. 68497-1-Ig
Clone No.2C1D7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

BMP type 2 receptor, BMP type II receptor, BMPR 2, BMPR II, BMPR2, BMPR3, BMR2, BRK 3, FLJ41585, FLJ76945, PPH1, T ALK

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, NIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68497-1-Ig targets BMPR2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BMPR2 fusion protein Ag5868 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type II (serine/threonine kinase)
Calculated molecular weight 115 kDa
Observed molecular weight120-140 kDa
GenBank accession numberBC052985
Gene Symbol BMPR2
Gene ID (NCBI) 659
RRIDAB_3085208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13873
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. Dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form and 150 kD dimer.

Protocols

Product Specific Protocols
WB protocol for BMPR2 antibody 68497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols