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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated BNIP3 Monoclonal ant (CL488-68091)

    IF Staining of HepG2 using CL488-68091

    Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using CoraLite® Plus 488 BNIP3 antibody (CL488-68091, Clone: 2H7D8 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated BNIP3 Monoclonal antibody

BNIP3 Monoclonal Antibody for IF/ICC
Cat No. CL488-68091
Clone No.2H7D8

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Synonyms

BNIP3, NIP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inCobalt Chloride treated HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68091 targets BNIP3 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BNIP3 fusion protein Ag27935 相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kDa interacting protein 3
Calculated molecular weight 194 aa, 22 kDa
Observed molecular weight25-30,50-60 kDa
GenBank accession numberBC009342
Gene Symbol BNIP3
Gene ID (NCBI) 664
RRIDAB_3084396
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12983
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BNIP3 antibody CL488-68091Download protocol
Standard Protocols
Click here to view our Standard Protocols