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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated BNIP3L Monoclonal an (CL488-68118)

    IF Staining of HeLa using CL488-68118

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 BNIP3L antibody (CL488-68118, Clone: 1F6A3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated BNIP3L Monoclonal an (CL488-68118)

    FC experiment of HeLa using CL488-68118

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated BNIP3L Monoclonal antibody (CL488-68118, Clone:1F6A3)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated BNIP3L Monoclonal antibody

BNIP3L Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68118
Clone No.1F6A3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

BNIP3H, BNIP3A, BNIP, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, Adenovirus E1B19K-binding protein B5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68118 targets BNIP3L in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BNIP3L fusion protein Ag31547 相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kDa interacting protein 3-like
Calculated molecular weight 24 kDa
Observed molecular weight35 kD
GenBank accession numberBC001559
Gene Symbol BNIP3L
Gene ID (NCBI) 665
RRIDAB_3672971
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60238
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BNIP3L, also named as BNIP3A, BNIP3H, NIX and NIP3L. Bnip3L is one of the BH3-containing pro-apoptotic proteins and that it targets the mitochondria when inducing apoptosis. It may function as a tumor suppressor. BNIP3L has Dimer form with MW ~70 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BNIP3L antibody CL488-68118Download protocol
FC protocol for CL Plus 488 BNIP3L antibody CL488-68118Download protocol
Standard Protocols
Click here to view our Standard Protocols