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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BORIS Monoclonal antibody, PBS Only

BORIS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12E2

Cat no : 60079-1-PBS

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of SMMC-7721 using 60079-1-Ig (same clone as 60079-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60079-1-PBS targets BORIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BORIS fusion protein Ag1003 相同性解析による交差性が予測される生物種
Full Name CCCTC-binding factor (zinc finger protein)-like
Calculated molecular weight 82 kDa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC130486
Gene symbol BORIS/CTCFL
Gene ID (NCBI) 140690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.