• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of SMMC-7721 using 60079-1-Ig (same clone as 60079-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    IF Staining of HeLa using 60079-1-Ig (same clone as 60079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60079-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

BORIS Monoclonal antibody, PBS Only

BORIS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60079-1-PBS
Clone No.2B12E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60079-1-PBS targets BORIS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BORIS fusion protein Ag1003 相同性解析による交差性が予測される生物種
Full Name CCCTC-binding factor (zinc finger protein)-like
Calculated molecular weight 82 kDa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC130486
Gene Symbol BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_1211604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NI51
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.