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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BORIS Monoclonal antibody

BORIS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12E2

Cat no : 60079-1-Ig

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, Zinc finger protein CTCF T

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of HeLa using 60079-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using BORIS Monoclonal antibody (60079-1-Ig)

    WB analysis of SMMC-7721 using 60079-1-Ig

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60079-1-Ig (BORIS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BORIS Monoclonal antibody (60079-1-Ig)

    IF Staining of HeLa using 60079-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BORIS antibody (60079-1-Ig, Clone: 2B12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHela cells, SMMC-7721 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60079-1-Ig targets BORIS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BORIS fusion protein Ag1003 相同性解析による交差性が予測される生物種
Full Name CCCTC-binding factor (zinc finger protein)-like
Calculated molecular weight 82 kDa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC130486
Gene symbol BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_1211604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Protocols

Product Specific Protocols
WB protocol for BORIS antibody 60079-1-IgDownload protocol
IF protocol for BORIS antibody 60079-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article