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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated BORIS Monoclonal antibody (CL594-60079)

    IF Staining of HeLa using CL594-60079

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 BORIS antibody (CL594-60079, Clone: 2B12E2 ) at dilution of 1:200.

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CoraLite®594-conjugated BORIS Monoclonal antibody

BORIS Monoclonal Antibody for IF/ICC
Cat No. CL594-60079
Clone No.2B12E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60079 targets BORIS in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BORIS fusion protein Ag1003 相同性解析による交差性が予測される生物種
Full Name CCCTC-binding factor (zinc finger protein)-like
Calculated molecular weight 82 kDa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC130486
Gene Symbol BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_2883418
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NI51
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

Protocols

Product Specific Protocols
IF protocol for CL594 BORIS antibody CL594-60079Download protocol
Standard Protocols
Click here to view our Standard Protocols