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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated BRE Monoclonal antibody

BRE Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E5A5

Cat no : CL594-66356

Synonyms

BRCC4, BRCA1-A complex subunit BRE, BRCA1/BRCA2-containing complex subunit 45, BRCA1 A complex subunit BRE, Brain and reproductive organ-expressed protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated BRE Monoclonal antibody (CL594-66356)

    IF Staining of mouse brain using CL594-66356

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 BRE antibody (CL594-66356, Clone: 2E5A5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated BRE Monoclonal antibody (CL594-66356)

    FC experiment of HeLa using CL594-66356

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated BRE Monoclonal antibody (CL594-66356, Clone:2E5A5)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66356 targets BRE in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BRE fusion protein Ag2256 相同性解析による交差性が予測される生物種
Full Name brain and reproductive organ-expressed (TNFRSF1A modulator)
Calculated molecular weight 383 aa, 44 kDa
Observed molecular weight 42-47 kDa
GenBank accession numberBC001251
Gene symbol BRE
Gene ID (NCBI) 9577
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 BRE antibody CL594-66356Download protocol
FC protocol for CL594 BRE antibody CL594-66356Download protocol
Standard Protocols
Click here to view our Standard Protocols