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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using BRF2 Polyclonal antibody (12056-1-AP)

    WB analysis of HT-1080 using 12056-1-AP (same clone as 12056-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using BRF2 Polyclonal antibody (12056-1-AP)

    WB analysis of DU 145 using 12056-1-AP (same clone as 12056-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12056-1-PBS in a different storage buffer formulation.

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BRF2 Polyclonal antibody, PBS Only

BRF2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12056-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12056-1-PBS targets BRF2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRF2 fusion protein Ag2686 相同性解析による交差性が予測される生物種
Full Name BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
Calculated molecular weight 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC010648
Gene Symbol BRF2
Gene ID (NCBI) 55290
RRIDAB_2218683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAW0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.