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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated BRPF1 Polyclonal ant (CL488-30186)

    FC experiment of HeLa using CL488-30186

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Brpf1 Polyclonal Antibody (CL488-30186)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated BRPF1 Polyclonal antibody

BRPF1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-30186

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration:  100 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30186 targets BRPF1 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRPF1 fusion protein Ag32828 相同性解析による交差性が予測される生物種
Full Name bromodomain and PHD finger containing, 1
Calculated molecular weight 1220 aa, 138 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC053851
Gene Symbol BRPF1
Gene ID (NCBI) 7862
RRIDAB_3672844
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55201
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 BRPF1 antibody CL488-30186Download protocol
Standard Protocols
Click here to view our Standard Protocols