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BSAP,PAX5 Monoclonal antibody
BSAP,PAX5 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, FC (Intra), IP, ELISA, IF
Conjugate
Unconjugated
CloneNo.
2H2D6
Cat no : 60349-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Ramos cells, Daudi cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
60349-1-Ig targets BSAP,PAX5 in WB, IHC, FC (Intra), IP, ELISA, IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BSAP,PAX5 fusion protein Ag16023 相同性解析による交差性が予測される生物種 |
| Full Name | paired box 5 |
| Calculated molecular weight | 391 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC156927 |
| Gene symbol | PAX5 |
| Gene ID (NCBI) | 5079 |
| RRID | AB_2881458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BSAP,PAX5 antibody 60349-1-Ig | Download protocol |
| IHC protocol for BSAP,PAX5 antibody 60349-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (03-28-2024) | This antibody reacted mouse lymphocytes in tonsil and worked well for immunolabeling.
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