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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BSAP,PAX5 Monoclonal antibody

BSAP,PAX5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H2D6

Cat no : CL594-60349

Synonyms

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Ramos cells using CoraLite®594-conjugated BSAP,PAX5 Monoclonal antib (CL594-60349)

    FC experiment of Ramos using CL594-60349

    1X10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human BSAP,PAX5 (CL594-60349, Clone:2H2D6) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inRamos cells
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60349 targets BSAP,PAX5 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BSAP,PAX5 fusion protein Ag16023 相同性解析による交差性が予測される生物種
Full Name paired box 5
Calculated molecular weight 391 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC156927
Gene symbol PAX5
Gene ID (NCBI) 5079
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Protocols

Product Specific Protocols
FC protocol for CL594 BSAP,PAX5 antibody CL594-60349Download protocol
Standard Protocols
Click here to view our Standard Protocols