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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated BTC Monoclonal antibody

BTC Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H5E8

Cat no : CL488-66683

Synonyms

betacellulin, BTC, Probetacellulin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated BTC Monoclonal antib (CL488-66683)

    WB analysis using CL488-66683

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66683 (BTC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66683 targets BTC in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BTC fusion protein Ag26724 相同性解析による交差性が予測される生物種
Full Name betacellulin
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011618
Gene symbol BTC
Gene ID (NCBI) 685
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 BTC antibody CL488-66683Download protocol
Standard Protocols
Click here to view our Standard Protocols