Featured Product
KD/KO Validated

BUB1B Polyclonal antibody

Name: BUB1B Polyclonal antibody
Brand: Proteintech
SKU: 11504-2-AP

BUB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11504-2-AP

Synonyms

BubR1, MAD3/BUB1-related protein kinase, hBUBR1, EC:2.7.11.1, BUB1beta

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of mouse testis using 11504-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11504-2-AP
WB result of BubR1 antibody (11504-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BubR1 transfected HeLa cells.
WB analysis of human testis using 11504-2-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11504-2-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11504-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11504-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11504-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 11504-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 11504-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11504-2-AP (BubR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11504-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 11504-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 11504-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 11504-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 11504-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).
IF Staining of SKOV-3 using 11504-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using BubR1 antibody (11504-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of SKOV-3 using 11504-2-AP
Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of SKOV-3 using 11504-2-AP
Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 11504-2-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, K-562 cells, HeLa cells, human testis tissue
Positive IHC detected in	human cervical cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SKOV-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 3 publications below
IF	See 4 publications below

Product Information

11504-2-AP targets BUB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BUB1B fusion protein Ag2053 相同性解析による交差性が予測される生物種
Full Name	budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Calculated molecular weight	1050 aa, 120 kDa
Observed molecular weight	120-130 kDa
GenBank accession number	BC018739
Gene symbol	BubR1
Gene ID (NCBI)	701
RRID	AB_2066073
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Protocols

Product Specific Protocols
WB protocol for BUB1B antibody 11504-2-AP	Download protocol
IHC protocol for BUB1B antibody 11504-2-AP	Download protocol
IF protocol for BUB1B antibody 11504-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint. Authors - Fengjie Yuan View Article
human	WB	Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. Authors - Zhi-Chuan Zhu View Article
human	WB	Cancer Lett Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells. Authors - Chuangyu Wen View Article
human	WB	Bioorg Chem Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors. Authors - Yuru Liang View Article
human	WB	Eur J Med Chem Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents. Authors - Pengfei Zhou View Article
human	WB	Int J Biol Sci The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. Authors - Wei Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BUB1B Polyclonal antibody

BUB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse testis using 11504-2-AP

WB analysis of HeLa using 11504-2-AP

WB analysis of human testis using 11504-2-AP

WB analysis of K-562 using 11504-2-AP

WB analysis of HeLa using 11504-2-AP

WB analysis of mouse testis using 11504-2-AP

WB analysis of mouse testis using 11504-2-AP

WB analysis of mouse testis using 11504-2-AP

IHC staining of human cervical cancer using 11504-2-AP

IHC staining of human colon cancer using 11504-2-AP

IHC staining of human colon cancer using 11504-2-AP

IHC staining of human colon cancer using 11504-2-AP

IHC staining of human colon cancer using 11504-2-AP

IHC staining of human colon cancer using 11504-2-AP

IF Staining of SKOV-3 using 11504-2-AP

IF Staining of SKOV-3 using 11504-2-AP

IF Staining of SKOV-3 using 11504-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.

Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors.

Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.