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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using CoraLite® Plus 488-conjugated BubR1 Polyclonal ant (CL488-11504)

    IF Staining of SKOV-3 using CL488-11504

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 BubR1 antibody (CL488-11504) at dilution of 1:100.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BubR1 Polyclonal antibody

BubR1 Polyclonal Antibody for IF/ICC
Cat No. CL488-11504

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

BUB1B, Bub1A, MAD3/BUB1-related protein kinase, hBUBR1, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11504 targets BubR1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BubR1 fusion protein Ag2053 相同性解析による交差性が予測される生物種
Full Name budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Calculated molecular weight 1050 aa, 120 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC018739
Gene Symbol BubR1
Gene ID (NCBI) 701
RRIDAB_3083903
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60566
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BubR1 antibody CL488-11504Download protocol
Standard Protocols
Click here to view our Standard Protocols