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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 488-conjugated Bcl-XL Polyclonal an (CL488-26967)

    IF Staining of NIH/3T3 using CL488-26967

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 Bcl-XL antibody (CL488-26967) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated Bcl-XL Polyclonal an (CL488-26967)

    FC experiment of Jurkat using CL488-26967

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Bcl-XL (CL488-26967) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated Bcl-XL Polyclonal antibody

Bcl-XL Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26967

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26967 targets Bcl-XL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag25604 相同性解析による交差性が予測される生物種
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
RRIDAB_2934419
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Bcl-XL antibody CL488-26967Download protocol
FC protocol for CL Plus 488 Bcl-XL antibody CL488-26967Download protocol
Standard Protocols
Click here to view our Standard Protocols