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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Bcl-XL Recombinant antibody (83330-1-RR)

    WB analysis using 83330-1-RR (same clone as 83330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Bcl-XL Recombinant antibody (83330-1-RR)

    IF Staining of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Bcl-XL Recombinant antibody (83330-1-RR)

    FC experiment of HeLa using 83330-1-RR (same clone as 83330-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83330-1-RR

    Affinity and Kinetic Characterization of 83330-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83330-1-RR against Human Bcl-XL were performed. The affinity constant is 0.792 nM.

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Bcl-XL Recombinant antibody, PBS Only

Bcl-XL Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83330-1-PBS
Clone No.1E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83330-1-PBS targets Bcl-XL in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bcl-XL fusion protein Ag25604 相同性解析による交差性が予測される生物種
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
GenBank accession numberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07817
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.