Bcl-XL Recombinant antibody

Bcl-XL Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E8

Cat no : 83330-1-RR

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092


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Tested Applications

Positive WB detected inA549 cells, HeLa cells, Ramos cells, K-562 cells, RAW 264.7 cells, C6 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83330-1-RR is Bcl-XL Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bcl-XL fusion protein Ag25604 相同性解析による交差性が予測される生物種
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight30 kDa
GenBank accession numberBC019307
Gene symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 83330-1-RRDownload protocol
IF protocol for Bcl-XL antibody 83330-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols