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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (30226-1-AP)

    WB analysis using 30226-1-AP (same clone as 30226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30226-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (30226-1-AP)

    WB analysis using 30226-1-AP (same clone as 30226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30226-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Beta-2-Microglobulin Polyclonal antibody (30226-1-AP)

    IF Staining of NCCIT using 30226-1-AP (same clone as 30226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Beta-2-Microglobulin antibody (30226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30226-1-PBS in a different storage buffer formulation.

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Beta-2-Microglobulin Polyclonal antibody, PBS Only

Beta-2-Microglobulin Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30226-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30226-1-PBS targets Beta-2-Microglobulin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight12-14 kDa
GenBank accession numberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61769
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.