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HRP-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB
Cat No. HRP-60008
Clone No.7D2C10

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, zebrafish, plant and More (5)

Applications

WB, IP, ELISA

Actin, ACTB, bate actin, β actin, Beta-actin

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, multi-cells/tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-60008 targets Beta Actin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivityhuman, mouse, rat, pig, canine, monkey, chicken, hamster
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene Symbol Beta Actin
Gene ID (NCBI) 60
RRIDAB_2819183
Conjugate HRP
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP60709
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Protocols

Product Specific Protocols
WB protocol for HRP Beta Actin antibody HRP-60008Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate

Authors - Kazushige Shiraishi

Gastroenterology

Dual inhibition of CDK4/6 and XPO1 induces senescence with acquired vulnerability to CRBN-based PROTAC drugs

Authors - Hui Wang
WB

J Hematol Oncol

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation

Authors - Meilin Xue
WB

Nat Neurosci

Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.

Authors - Kornélia Szebényi
mouseWB

Blood

Fetal liver hepcidin secures iron stores in utero.

Authors - Lara Kämmerer
humanWB

Nat Microbiol

Adeno-associated virus 2 bound to its cellular receptor AAVR.

Authors - Ran Zhang
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