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  • Featured Product
  • KD/KO Validated

Beta Catenin Monoclonal antibody, PBS Only

Beta Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B8A1

Cat no : 66379-1-PBS

Synonyms

Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Catenin Monoclonal antibody (66379-1-Ig)

    WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Beta Catenin Monoclonal antibody (66379-1-Ig)

    WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (beta-Catenin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)

    IP result of anti-Beta Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of rat colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of rat colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human liver cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human liver cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human breast cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human breast cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IHC staining of human colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IF Staining of human colon cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IF Staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IF Staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Beta Catenin Monoclonal antibody (66379-1-Ig)

    IF Staining of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Beta Catenin Monoclonal antibody (66379-1-Ig)

    FC experiment of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66379-1-PBS targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberNM_001904
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
RRIDAB_2857358
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)