Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (beta-Catenin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)
IP result of anti-Beta Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66379-1-Ig (same clone as 66379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IF Staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IF Staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
FC experiment of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)
1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.
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1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Beta Catenin Monoclonal antibody, PBS Only

Beta Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 66379-1-PBS

Clone No.1B8A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66379-1-PBS targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight	781 aa, 86 kDa
Observed molecular weight	92 kDa
GenBank accession number	NM_001904
Gene Symbol	Beta Catenin
Gene ID (NCBI)	1499
ENSEMBL Gene ID	ENSG00000168036
RRID	AB_2857358
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P35222
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of rat colon using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of rat colon using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of human ovary cancer using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of human breast cancer using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of human breast cancer using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of human colon using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)

IHC staining of human colon using 66379-1-Ig (same clone as 66379-1-PBS)

IF Staining of human colon cancer using 66379-1-Ig (same clone as 66379-1-PBS)

IF Staining of human skin cancer using 66379-1-Ig (same clone as 66379-1-PBS)