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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 647-conjugated Beta Catenin Polyclo (CL647-51067)

    IF Staining of MCF-7 using CL647-51067

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 Beta Catenin antibody (CL647-51067) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 647-conjugated Beta Catenin Polyclo (CL647-51067)

    FC experiment of MCF-7 using CL647-51067

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Beta Catenin (CL647-51067) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Beta Catenin Polyclo (CL647-51067)

    FC experiment of HeLa using CL647-51067

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Beta Catenin (CL647-51067) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-51067

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-51067 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2920240
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Beta Catenin antibody CL647-51067Download protocol
Standard Protocols
Click here to view our Standard Protocols