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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Beta Galactosidase M (CL488-66586)

    IF Staining of HepG2 using CL488-66586

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 Beta Galactosidase antibody (CL488-66586, Clone: 4F4F4 ) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Beta Galactosidase Monoclonal antibody

Beta Galactosidase Monoclonal Antibody for IF/ICC
Cat No. CL488-66586
Clone No.4F4F4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66586 targets Beta Galactosidase in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Beta Galactosidase fusion protein Ag8069 相同性解析による交差性が予測される生物種
Full Name galactosidase, beta 1
Calculated molecular weight 76 kDa
Observed molecular weight 64-66 kDa, 76-85 kDa
GenBank accession numberBC007493
Gene Symbol GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_2919348
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16278
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Beta Galactosidase antibody CL488-66586Download protocol
Standard Protocols
Click here to view our Standard Protocols