Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)Affinity

WB analysis using 80713-1-RR (same clone as 80713-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)
WB result of Beta Tubulin antibody (80713-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Tubulin transfected HEK-293 cells. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
WB analysis of zebrafish using 80713-1-RR (same clone as 80713-1-PBS)
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)
IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IF Staining of mouse eye using 80713-1-RR (same clone as 80713-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 80713-1-RR (same clone as 80713-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)
1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
×
1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80713-1-RR
Biolayer interferometry (BLl) kinetic assays of 80713-1-RR against Human Beta Tubulin were performed. The affinity constant is below 1 pM.

Featured Product
KD/KO Validated

Beta Tubulin Recombinant antibody, PBS Only

Beta Tubulin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Cat No. 80713-1-PBS

Clone No.2O13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80713-1-PBS targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Beta Tubulin fusion protein Ag0117 相同性解析による交差性が予測される生物種
Full Name	tubulin, beta 3
Calculated molecular weight	450 aa, 50 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC000748
Gene Symbol	TUBB3
Gene ID (NCBI)	10381
RRID	AB_2918906
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13509
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 80713-1-RR (same clone as 80713-1-PBS)

WB analysis of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

WB analysis of zebrafish using 80713-1-RR (same clone as 80713-1-PBS)

IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)

IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)

IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

IF Staining of mouse eye using 80713-1-RR (same clone as 80713-1-PBS)

IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)

IF Staining of HepG2 using 80713-1-RR (same clone as 80713-1-PBS)

IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

Affinity and Kinetic Characterization of 80713-1-RR

Beta Tubulin Recombinant antibody, PBS Only

Beta Tubulin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Beta Tubulin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA