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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta Tubulin Recombinant antibody, PBS Only

Beta Tubulin Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2O13

Cat no : 80713-1-PBS

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Tubulin Recombinant antibody (80713-1-RR)

    WB analysis using 80713-1-RR (same clone as 80713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    WB analysis of zebrafish using 80713-1-RR (same clone as 80713-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IP experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

    IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of human colon using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of rat brain using 80713-1-RR (same clone as 80713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of mouse eye using 80713-1-RR (same clone as 80713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of C2C12 using 80713-1-RR (same clone as 80713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of HepG2 using 80713-1-RR (same clone as 80713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of HeLa using 80713-1-RR (same clone as 80713-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    FC experiment of HEK-293 using 80713-1-RR (same clone as 80713-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80713-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80713-1-RR

    Affinity and Kinetic Characterization of 80713-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80713-1-RR against Human Beta Tubulin were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80713-1-PBS targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Tubulin fusion protein Ag0117 相同性解析による交差性が予測される生物種
Full Name tubulin, beta 3
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC000748
Gene symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.