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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Beta Tubulin Polyclonal an (CL594-10068)

    IF Staining of HeLa using CL594-10068

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Beta Tubulin antibody (CL594-10068) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Beta Tubulin Polyclonal an (CL594-10068)

    FC experiment of HeLa using CL594-10068

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Beta Tubulin (CL594-10068) (red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-10068

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10068 targets Beta Tubulin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Tubulin fusion protein Ag0117 相同性解析による交差性が予測される生物種
Full Name tubulin, beta 3
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2934698
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13509
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Protocols

Product Specific Protocols
IF protocol for CL594 Beta Tubulin antibody CL594-10068Download protocol
Standard Protocols
Click here to view our Standard Protocols