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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HRP-conjugated Beta Tubulin Recombinant antibody (HRP-80713)

    WB analysis using HRP-80713

    Various lysates were subjected to SDS PAGE followed by western blot with HRP-80713 (Beta Tubulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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HRP-conjugated Beta Tubulin Recombinant antibody

Beta Tubulin Uni-rAbTM Recombinant Antibody for WB
Cat No. HRP-80713
Clone No.2O13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-80713 targets Beta Tubulin in WB applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Tubulin fusion protein Ag0117 相同性解析による交差性が予測される生物種
Full Name tubulin, beta 3
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_3673981
Conjugate HRP
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13509
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Protocols

Product Specific Protocols
WB protocol for HRP Beta Tubulin antibody HRP-80713Download protocol
Standard Protocols
Click here to view our Standard Protocols