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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bif-1 Recombinant antibody

Bif-1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240623E1

Cat no : 83715-1-RR

Synonyms

SH3GLB1, Endophilin-B1, Endophilin B1, Bif1, Bax-interacting factor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Bif-1 Recombinant antibody (83715-1-RR)

    WB analysis using 83715-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83715-1-RR (Bif-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Bif-1 Recombinant antibody (83715-1-RR)

    IF Staining of HeLa using 83715-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bif-1 antibody (83715-1-RR, Clone: 240623E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83715-1-RR

    Affinity and Kinetic Characterization of 83715-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83715-1-RR against Human Bif-1 were performed. The affinity constant is 0.71 nM.

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83715-1-RR is Bif-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bif-1 fusion protein Ag7710 相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like endophilin B1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC007455
Gene symbol Bif-1
Gene ID (NCBI) 51100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).

Protocols

Product Specific Protocols
WB protocol for Bif-1 antibody 83715-1-RRDownload protocol
IF protocol for Bif-1 antibody 83715-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols