Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83715 targets Bif-1 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Bif-1 fusion protein Ag7710 相同性解析による交差性が予測される生物種 |
| Full Name | SH3-domain GRB2-like endophilin B1 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC007455 |
| Gene Symbol | Bif-1 |
| Gene ID (NCBI) | 51100 |
| RRID | AB_3673294 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y371 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Bif-1 antibody CL488-83715 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |